Operation And Calibration of Micropipetter

1.0       OBJECTIVE

                  To lay down a procedure for operation and calibration of micropipetter.

2.0        RESPONSIBILTY

            Microbiologist / Q.C Executive

3.0        ACCOUNTABILITY                        

            Head - Quality Control

4.0        PROCEDURE

4.1   For 0-200ml micropipetter.  (carry out all the activities at Temperature 25°C  )

4.1.1     Adjust micropipetter to 50ml by rotating the adjusting scale at clockwise or anticlockwise direction.

4.1.2     Insert the micro-tip tightly to the micropipetter.

4.1.3     Pipette out in previously tared beaker on weighing balance and note the reading.

4.1.4     Carry out the procedure twice and note the weight displayed on the balance, calculate the mean and standard deviation of three readings and record it in the format as given in annexure-I.

4.1.5          The weight per ml of water at 25°C when weight in air is 0.99602 g .Divide the mean with weight per ml to get the volume dispensed.

4.1.6          Carry out similar procedure by setting micropipetter to 100ml and 200ml .

4.2         For 200ml to 1000ml micropipetter

4.2.1     Repeat the same procedure as above by setting 200, 500 & 1000ml quantity.

4.2.2     Note the observations in the format given in Annexure-I

4.3        ACCEPTANCE CRITERIA :

             4.3.1    Standard deviation between three readings should not be more than 1%.     

                        4.3.2     The volume dispensed should not vary by more than 1% of the set volume.

 4.4       FREQUENCY OF CALIBRATION

             

Quarterly