Depyrogenation Of Apparatus

• PROCEDURE:

Ø      Lab apparatus used for LAL testing or sterile material sampling should be cleaned and thoroughly rinsed with distilled water. Thereafter dried and wrapped in double aluminum foil.

Ø      Set the hot air oven at 250°C , wait till the temperature reaches up to the set temperature.

Ø      After reaching the set temperature keep the aluminum foil wrapped apparatus inside the oven for not less than one hour.                                   

Ø      After completion of depyrogenation cycle , switch off the power supply and allow the oven temperature to reach  room temperature and then take out the apparatus carefully.

Note down the date , time , name of apparatus , done by and checked by in the format given in Annexure -1

De-Pyrogenation of Apparatus

Date	Operation time		Holding time/ Temp.	Item sterilized	Done  by	Check by
	From: hrs.	To:  hrs
			60 min / 250 °C	Sampling bottles, Glass-petri plates,Test-tubes for testing BET,Spatula,Forceps,Scissor,Vial opener Others-