Friday, December 17, 2010
Environmental Monitoring In Microbiology Laboratory
· PROCEDURE :
Ø The environmental monitoring of the area shall be carried out by settle plates.
Ø The Personnel monitoring shall be carried out by taking finger dabs and garment samples using contact plates.
Ø The Physical parameters like Temperature, Humidity and Differential pressure in the area shall be monitored.
· MONITORING BY SETTLE-PLATE METHOD
Ø Enter the different areas following the appropriate gowning practices.
Ø Expose the pre-incubated sterile media plates at different locations shown in annexure-I.
Ø Collect the plates after completing exposure hrs and incubate at 20-25°C for 72 hrs followed by 30-35°C for 48 hrs.
Ø The time of exposure, limits, frequency of monitoring applicable as per various grades and is attached in annexure II.
Ø Record the counts in the format as mentioned in annexure III.
Ø If the bio-burden is found out of the specified limit, proceed
· PROCEDURE FOR PERSONNEL MONITORING
Ø Transfer pre-incubated contact plates containing sterile SCDA media and the hand impressions and garment contacts of technician and helpers are taken at the place of operation.
Ø After taking the samples close the lid of the petri –plate and write the name of the person, nature of work and date & time at the base of the plate.
Ø Transfer those plates to the incubator having temperature set at 20-25°C for 72 hrs followed by 30-35°C for 48 hrs.
Ø Observe the plates after completion of incubation period and note the observations in the format as mentioned in annexure IV.
Ø If the bio-burden is found out of the specified limit, proceed.Monitor, physical parameters like temperature, humidity and pressure differential at critical places, once a day, in microbiology laboratory in respective formats / Register.